Effects of Probiotics on Mouse Adipose-Derived MSCs
In this study, two probiotic strains, Lacticaseibacillus rhamnosus and Lactobacillus delbrueckii, were tested on mouse adipose-derived mesenchymal stem cells. The results showed that these probiotics enhanced the proliferation of these cells and reduced cell death. They also influenced cytokine expression, decreasing pro-inflammatory cytokines and increasing anti-inflammatory mediators, while the frequency of regulatory T cells significantly increased.
- Tags
- #MSResearch
- Most relevant to
- —
- Human efficacy
- Not yet tested in people
- Mechanism
- Not reported
- Evidence strength
- Early – laboratory or animal research
- Outcome
- Not reported
Results in cells or animals often do not carry over to people with MS.
Why it matters
These findings suggest that probiotics may modulate immune responses by impacting the behavior of adipose-derived stem cells and could inform future research on treatments related to immune regulation.
What this does not prove
However, data is based on mRNA expression, not validated at the protein level. This means the changes observed are indicative rather than confirming actual immune responses, requiring caution in interpretation.
Next milestone
No next milestone was established from the available source.
Study facts
- Study design
- Not reported
- Participants / samples
- Not reported
- Randomised
- Not reported
- Controlled
- Not reported
- Primary endpoint met
- Not reported
- Relevant MS type
- Not reported
- Publication date
- 2026-10-21
- Evidence reviewed
- Abstract only
- Regulatory approval
- Not reported
- Research areas
- Not classified
- Biological target
- AD-MSCs, Tregs, Cytokines
- Pathway
- Not reported
- Population
- Not reported
- Size of effect
- Not reported
- Secondary endpoints
- Not reported
- MRI endpoints
- Not reported
- Biomarkers
- Not reported
- Safety
- Not reported
Original sources
Supporting passages (12)
subjectsCells
publication date2026-10-21
interventionLacticaseibacillus rhamnosus and Lactobacillus delbrueckii
primary endpointThis study evaluated the effects of Lacticaseibacillus rhamnosus (formerly Lactobacillus rhamnosus) and Lactobacillus delbrucekii on the immunomodulatory properties and cytokine-expression profile of adipose-derived MSCs (AD-MSCs) isolated from patients with MS.
findingsBoth probiotic candidate strains, particularly at 107, significantly enhanced AD-MSC proliferation and reduced apoptosis, the mRNA expression of pro-inflammatory cytokines IL6 and IL17 were downregulated in all treatment groups except the low-dose L.
findingsmRNA expression of pro-inflammatory cytokines IL6 and IL17 were downregulated in all treatment groups except the low-dose L. rhamnosus group.
findingsIn contrast, the mRNA expression of anti-inflammatory mediators TGFβ, IL4, and FOXP3 were upregulated following treatment with the probiotic candidate strains.
findingsTreg frequency increased significantly in all groups (P = 0.0001).
limitationsHowever, because these conclusions are based on mRNA expression data without protein-level validation, they should be interpreted cautiously and regarded as indicative of transcriptional changes rather than confirmed cytokine or functional immune responses.
biological targetsFollowing 24 h of co-culture of AD-MSCs with peripheral blood mononuclear cells (PBMCs) from healthy donors in the presence of probiotic candidate strains, the frequency of regulatory T cells (Tregs) and expression of IL1, IL6, IL17, FOXP3, TGFβ, and IL4 were evaluated by flow cytometery and real-time PCR, respectively.
peer reviewedPublication types: Journal Article
human efficacyCells
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