Liproxstatin-1 in Female C57BL/6 Mice with EAE
In a study with female C57BL/6 mice with experimental autoimmune encephalomyelitis (EAE), early treatment with Liproxstatin-1 reduced clinical severity at day 30 compared to EAE controls (p < 0.001) and limited body weight loss. Imaging showed that treatment attenuated the disease-related changes in brain microstructure, such as reduced diffusivity and persistent fractional anisotropy loss in the white matter.
- Tags
- #MSResearch
- Most relevant to
- —
- Human efficacy
- Not yet tested in people
- Mechanism
- Not reported
- Evidence strength
- Early – laboratory or animal research
- Outcome
- Not reported
Results in cells or animals often do not carry over to people with MS.
Why it matters
This research suggests that early intervention with Liproxstatin-1 may offer protective effects against neurological damage in EAE, indicating potential insights into the mechanisms of multiple sclerosis and its treatment strategies.
What this does not prove
The study does not establish the long-term effects of the treatment beyond the follow-up period. Also, results in mice may not directly translate to human multiple sclerosis outcomes.
Next milestone
No next milestone was established from the available source.
Study facts
- Study design
- Not reported
- Participants / samples
- Not reported
- Randomised
- Not reported
- Controlled
- Not reported
- Primary endpoint met
- Not reported
- Relevant MS type
- Not reported
- Publication date
- 2026-10-24
- Evidence reviewed
- Abstract only
- Regulatory approval
- Not reported
- Research areas
- Not classified
- Biological target
- Acyl-CoA synthetase long-chain family member 4 (ACSL4), Glutathione peroxidase 4 (GPX4)
- Pathway
- Not reported
- Population
- female C57BL/6 mice with EAE induced by MOG35-55
- Size of effect
- p < 0.001
- Secondary endpoints
- Not reported
- MRI endpoints
- Diffusion tensor imaging (DTI)
- Biomarkers
- Not reported
- Safety
- Not reported
Original sources
- Primary evidenceDiffusion MRI reveals stage-dependent microstructural responses to early ferroptosis inhibition in experimental autoimmune encephalomyelitis. ↗DOI: 10.1016/j.bbi.2026.107084
Supporting passages (18)
sample size basisfemale C57BL/6 mice
publication date2026-10-24
interventionLiproxstatin-1 (Lip-1)
comparatorSixty-four female C57BL/6 mice were assigned to control, EAE, early-treatment (Early-Tx; Lip-1 from day 12), and late-treatment (Late-Tx; Lip-1 from day 18) groups.
primary endpointRESULTS: Early-Tx reduced clinical severity at day 30 (vs.
findingsEarly-Tx reduced clinical severity at day 30 (vs. EAE; p < 0.001)
findingsEAE; p < 0.001) and limited body weight loss.
findingsAt peak disease, voxel- and ROI-based DTI showed increased mean diffusivity and radial diffusivity in the corpus callosum, internal capsule, and thalamus, which were attenuated by Early-Tx.
findingsAt the chronic stage, EAE showed persistent fractional anisotropy reduction in periventricular white matter and corpus callosum, whereas Early-Tx largely prevented these abnormalities.
biological targetsAcyl-CoA synthetase long-chain family member 4 (ACSL4)
biological targetsAcyl-CoA synthetase long-chain family member 4 (ACSL4) upregulation and glutathione peroxidase 4 (GPX4) downregulation preceded overt iron deposition, whereas iron accumulation emerged mainly at the chronic stage and colocalized with activated microglia and demyelinated lesions.
populationMETHODS: We combined 7.0 T diffusion tensor imaging (DTI) with histopathological and molecular analyses to evaluate the effects of Liproxstatin-1 (Lip-1) in mice with EAE induced by myelin oligodendrocyte glycoprotein peptide 35-55 (MOG35-55).
mri endpointsMETHODS: We combined 7.0 T diffusion tensor imaging (DTI) with histopathological and molecular analyses to evaluate the effects of Liproxstatin-1 (Lip-1) in mice with EAE induced by myelin oligodendrocyte glycoprotein peptide 35-55 (MOG35-55).
effect sizep < 0.001
limitationsTitle: Diffusion MRI reveals stage-dependent microstructural responses to early ferroptosis inhibition in experimental autoimmune encephalomyelitis.
peer reviewedPublication types: Journal Article
subjectsTitle: Diffusion MRI reveals stage-dependent microstructural responses to early ferroptosis inhibition in experimental autoimmune encephalomyelitis.
human efficacyTitle: Diffusion MRI reveals stage-dependent microstructural responses to early ferroptosis inhibition in experimental autoimmune encephalomyelitis.
AI assessment, not yet reviewed by a person · version 1 · Community votes are separate from evidence review.