Human Subjects Study on CD4+ T Cells Transformation
A study involving three healthy donors and three multiple sclerosis patients examined the transformation of CD4+ T cells into regulatory T cells (Tregs). The transformed Tregs (tTregs) showed increased levels of the full-length FoxP3 splice variant and enhanced proliferative and suppressive activities compared to expanded Tregs (eTregs).
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Why it matters
This research highlights differences in Treg generation methods, with tTregs showing potential advantages in terms of FoxP3 variant expression and functional capabilities. However, these findings are laboratory-based and do not address clinical outcomes.
What this does not prove
The study does not establish clinical outcomes as it focuses solely on laboratory findings regarding Treg transformation and expansion.
Next milestone
No next milestone was established from the available source.
Study facts
- Study design
- Not reported
- Participants / samples
- 6 · Three healthy donors and three patients with multiple sclerosis
- Randomised
- Not reported
- Controlled
- Not reported
- Primary endpoint met
- Not reported
- Relevant MS type
- Not reported
- Publication date
- Not reported
- Evidence reviewed
- Abstract only
- Regulatory approval
- Not reported
- Research areas
- Not classified
Original sources
- Primary evidenceInduction of the Full-Length FoxP3 Splice Variant during ex vivo Transformation of Human CD4+ T Lymphocytes into Regulatory T Cells. ↗DOI: 10.1134/s0006297926601954
Supporting passages (8)
subjectsHuman
sample sizeIn this study, we used cells obtained from three healthy donors and three patients with multiple sclerosis to compared the levels of FoxP3 splice variants in Tregs during their ex vivo expansion (expanded Tregs, eTregs) and Tregs generated by transformation of CD4+ T cells (transformed Tregs, tTregs).
sample size basisIn this study, we used cells obtained from three healthy donors and three patients with multiple sclerosis to compared the levels of FoxP3 splice variants in Tregs during their ex vivo expansion (expanded Tregs, eTregs) and Tregs generated by transformation of CD4+ T cells (transformed Tregs, tTregs).
interventionTitle: Induction of the Full-Length FoxP3 Splice Variant during ex vivo Transformation of Human CD4+ T Lymphocytes into Regulatory T Cells.
comparatorIn contrast, ex vivo expansion of eTregs did not alter the proportions of FoxP3 splice variants; these cells proliferated less actively and exhibited lower suppressive activity than tTregs.
primary endpointOur findings demonstrate that transformation of CD4+ T cells into Tregs is accompanied by an increase in the proportion of the full-length FoxP3 splice variant.
findingsOur findings demonstrate that transformation of CD4+ T cells into Tregs is accompanied by an increase in the proportion of the full-length FoxP3 splice variant.
limitationsTitle: Induction of the Full-Length FoxP3 Splice Variant during ex vivo Transformation of Human CD4+ T Lymphocytes into Regulatory T Cells.
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